Spike S1, Recombinant, SARS-CoV-2, aa16-685, Avi-His-Tag (SARS-CoV-2)

Katalog-Nummer 544476-100ug

Size : 100ug

Marke : US Biological

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544476 Spike S1, Recombinant, SARS-CoV-2, aa16-685, Avi-His-Tag (SARS-CoV-2)

Clone Type
Polyclonal
Swiss Prot
P0DTC2
Grade
Purified
Accession #
QHD43416.1
Shipping Temp
Dry Ice
Storage Temp
-70°C

Recombinant protein corresponding to aa16-685 from Severe acute respiratory Coronavirus 2 Spike Glycoprotein S1 (SARS-CoV-2 Spike S1), fused to a C-terminal Avi-His-Tag, expressed in a HEK293 cells.||Molecular Weight:|~78kD, runs at a higher MW by SDS-PAGE due to glycosylation.||Assay Conditions:|SARS-CoV-2 Spike S1 protein was coated on the plate for overnight at 4°C, and tested for the binding to ACE2 using ACE2-His, Avi-Biotin labeled at various concentration. Streptavidin-HRP was used to develop the luminescence signal.||Applications: |Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications have not been tested. ||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Source: Recombinant, HEK293 cells|Purity: ≥90% (SDS-PAGE)|Concentration: ~1mg/ml|Form: Supplied as a liquid in PBS, pH 7.4, 20% glycerol.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in PBS, pH 7.4, 20% glycerol.
Purity
≥90% (SDS-PAGE)
References
1. Hoffman, M. et al., Cell. 2020 Apr; 181:1-10.|2. Walls, A. C. et al., Cell. 2020 Mar; 180(2):281-292.