SARS-CoV-2 (COVID-19) Alpha Variant (B.1.1.7, UK) Spike P681H

Katalog-Nummer 583257-100ug

Size : 100ug

Marke : US Biological

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583257 SARS-CoV-2 (COVID-19) Alpha Variant (B.1.1.7, UK) Spike P681H

Clone Type
Polyclonal
Host
rabbit
Swiss Prot
QHD43416
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Shipping Temp
Blue Ice
Storage Temp
-20°C

In September of 2020 a new lineage of SARS-CoV-2, known as B.1.1.7, was discovered in the United Kingdom. This lineage was found to have developed 14 lineage-specific amino acid replacements and 3 deletions prior to its discovery. The transmission of UK variant (B.1.1.7 lineage) was increased at least 50%. Increased severity and higher death rate were also found in UK variant. UK variant will not affect the effectiveness of COVID19 vaccine. One of the mutations associated with this lineage is a N501Y in the spike protein of the virus. It is believed that this mutation is able to increase the spike protein's affinity for the host ACE2 receptor and it has been associated with increased infectivity and virulence. B.1.1.7 viruses have also been shown to have a P681H in the cleavage site of spike protein. This location is one of the residues that make up the furin cleavage site between S1 and S2 in spike protein.||Applications: |Suitable for use in ELISA and Western Blot. Other applications not tested.||Recommended Dilution:|Western Blot: 1ug/ml|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Concentration: ~1mg/ml|Form: Supplied as a liquid in PBS, 0.02% sodium azide.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Synthetic peptide corresponding to aa650-700 near the center of SARS-CoV-2 UK variant (B.1.1.7) Spike protein. |Specificity: Recognizes SARS-CoV-2 (COVID-19) Alpha Variant (B.1.1.7, UK) Spike P681H. Cross-reactivity: SARS-CoV Spike proteins: (25%). May cross-react with several virus of interest (VOI) variant lineages that contains P681H mutation, including B.11.318, B.1.621, B.1.621.1, P.3. But all of these lineages are rarely present in current pandemic.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Synthetic peptide corresponding to aa650-700 near the center of SARS-CoV-2 UK variant (B.1.1.7) Spike protein.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes SARS-CoV-2 (COVID-19) Alpha Variant (B.1.1.7, UK) Spike P681H. Cross-reactivity: SARS-CoV Spike proteins: (25%). May cross-react with several virus of interest (VOI) variant lineages that contains P681H mutation, including B.11.318, B.1.621, B.1.621.1, P.3. But all of these lineages are rarely present in current pandemic.
References
1. Duchene et al. Virus Evolution 6(2): veaa061.|2. Gu et al. Science 369(6511):1603-1607|3. Hoffmann et al. Molecular Cell 78(4):779-784.e5|4. Davies et al. Science 372(6538):eabg3055.|5. Davies et al. Nature. 593(7858):270-274.|6. Graham, et al. The Lancet Public Health. 6(5): e335-e345.|7. Horby et al. New & Emerging Threats Advisory Group. 2020;91:264-266.