Rat RUNX2 (Runt Related Transcription Factor 2) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	Runx2Uniprot ID=RUNX2_RAT"
Specificity:	This kit recognizes Rat RUNX2 in samples. No significant cross-reactivity or interference between Rat RUNX2 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Phosphorylated.probably by MAP kinases (MAPK). Phosphorylation by HIPK3 is required for the SPEN/MINT and FGF2 transactivation during osteoblastic differentiation.
Function	Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. Inhibits KAT6B-dependent transcriptional activation. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).
Protein Name	Runt-Related Transcription Factor 2 Core-Binding Factor Subunit Alpha-1 Cbf-Alpha-1 Osteoblast-Specific Transcription Factor 2 Osf-2
Cellular Localisation	Nucleus Cytoplasm
Alternative CLIA Names	Runt-Related Transcription Factor 2 CLIA kit Core-Binding Factor Subunit Alpha-1 CLIA kit Cbf-Alpha-1 CLIA kit Osteoblast-Specific Transcription Factor 2 CLIA kit Osf-2 CLIA kit Runx2 CLIA kit Cbfa1 CLIA kit Osf2 CLIA kit
Specificity	This kit recognizes Rat RUNX2 in samples. No significant cross-reactivity or interference between Rat RUNX2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	96-111	104
EDTA plasma(n=8)	92-105	100
Cell culture media(n=8)	86-100	93

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	192.14	626.83	1569.76	187.48	569.38	1437.64
Standard deviation	19.16	51.15	106.43	22.89	47.03	98.62
CV (%)	9.97	8.16	6.78	12.21	8.26	6.86

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance