Rat IgM (Mu chain specific) rabbit polyclonal antibody, FITC
Specifications
Product Data
Recommended Dilution
Suitable for Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivity
Rat
Antibody Host
Rabbit
Clonality
Polyclonal
Immunogen
Rat IgM µ mu chain.
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Rat IgM and Rat Serum. No reaction was observed against Rat IgG or Rat IgA. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Rat heavy or light chain isotypes.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: FITC State: Lyophilized purified Ig fraction. Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons) Absorption emission: 495 nm / 528 nm Molar radio: 3.5 moles FITC per mole of Rabbit IgG.
Reconstitution Method
Restore with 1.0 ml of deionized water (or equivalent).
Concentration
lot specific
Purification
Immunoaffinity chromatography.
Conjugation
FITC
Storage
Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
