Western blot analysis of BSEP expression in LO2 (A), mouse pancreas (B) whole cell lysates. (Predicted band size: 146 kD; Observed band size: 160 kD)

Immunofluorescent analysis of BSEP staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).