Nanog (Early Embryo Specific Expression NK Family Gene, Embryonic Stem Cell Specific Homeobox Protein, ENK, Homeobox Transcription Factor Nanog, Nanog Homeobox)
Katalog-Nummer N0019-01L-100ul
Size : 100ul
Marke : US Biological
N0019-01L Rabbit Anti-Nanog (Early Embryo Specific Expression NK Family Gene, Embryonic Stem Cell Specific Homeobox Protein, ENK, Homeobox Transcription Factor Nanog, Nanog Homeobox)
Clone Type
PolyclonalHost
mouseSource
humanSwiss Prot
Q9H9S0Isotype
IgG1Grade
SupernatantApplications
FC IF IHC WBCrossreactivity
HuShipping Temp
Blue IceStorage Temp
-20°CNanog is a homeodomain containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc and Lin28 (2,3). ||Applications:|Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry and Immunofluorescence. Other applications not tested.||Recommended Dilution:|Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. |Immunofluorescence (IF-IC): 1:2000|Immunohistochemistry (paraffin): 1:12,800|Flow Cytometry: 1:1600|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.