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> IgM, Human Fc5u (Biotin)

IgM, Human Fc5u (Biotin)

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Katalog-Nummer I1903-38K-500ug

Size : 500ug

Marke : US Biological

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Telefonnummer : +1 850 650 7790

I1903-38K IgM, Human Fc5u (Biotin)

Clone Type
Polyclonal
Host
goat
Source
human
Isotype
F(ab’)2
Grade
Affinity Purified
Applications
E IC IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications: |Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications have not been tested.||Recommended Dilution: |ELISA: 1:50,000-1:1,000,000 |Western Blot: 1:10,000-1:100,000 |Immunohistochemistry (tissue sections)1:500-1:5000|Optimal dilutions to be determined by the researcher.||Label: |Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)||Biotin/Protein Ratio: |10-20 BAC molecules per Goat IgG molecule||Storage and Stability:|Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Product Type: Pab|Isotype: F(ab’)2|Host: goat|Source: human|Concentration: ~1mg/ml (after reconstitution) |Form: Supplied as a lyophilized powder from PBS, pH 7.2, 15 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free, 0.05% Sodium Azide, 0.01% Thimerosal. Labeled with Biotin.|Purity: Purified by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. |Immunogen: Human IgM Fc5µ fragment|Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Human IgM Fc5µ and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or other human heavy or light chain proteins.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Human IgM Fc5µ fragment
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 15 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free, 0.05% Sodium Azide, 0.01% Thimerosal. Labeled with Biotin.
Purity
Purified by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Human IgM Fc5µ and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or other human heavy or light chain proteins.