ASM10198-A700-100 | HCN4 Antibody Biotrend

Immunohistochemistry analysis using Mouse Anti-HCN4 Monoclonal Antibody, Clone S114-10 (ASM10198). Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody (ASM10198) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Western Blot analysis of Human T-HEK cell lysate showing detection of HCN4 protein using Mouse Anti-HCN4 Monoclonal Antibody, Clone S114-10 (ASM10198). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody (ASM10198) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunohistochemistry analysis using Mouse Anti-HCN4 Monoclonal Antibody, Clone S114-10 (ASM10198). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody (ASM10198) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	WB, IHC, AM
Primary Accession	Q9JKA7
Other Accession	NP_067690.1
Host	Mouse
Isotype	IgG1
Reactivity	Human, Mouse, Rat, Rabbit
Clonality	Monoclonal
Description	Mouse Anti-Rat HCN4 Monoclonal IgG1
Target/Specificity	Detects ~130kDa. No cross-reactivity against other HCNs.
Other Names	Hyperpolarization activated cyclic nucleotide gated potassium channel 4 Antibody, HCN 4 Antibody, Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 4 Antibody
Clone Names	S114-10
Immunogen	Fusion protein amino acids 1-019-1198 (c-terminus) of rat HCN4
Purification	Protein G Purified
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	1 µg/ml of SMC-320 was sufficient for detection of HCN4 in 10 µg of COS cell lysate transiently transfected with HCN4 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization	Membrane

Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abcepta antibody to
info@abcepta.com, and receive a free "I Love Antibodies" mug.