| Quantity | 100 µl |
|---|---|
| Antibody Type | Polyclonal |
| Host | Guinea pig |
| Conjugate | Unconjugated |
| Application | ICC/IF, IHC, WB |
| Purification | Stabilized antiserum |
| Reactivity | Human, Mouse |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Immunogen | Synthetic peptide (KTT EYQ LST LEE RD - C) of human keratin K20 (formerly also designated cytokeratin 20), coupled to KLH |
| Formulation | Contains 0.09% sodium azide and 0.5% BSA |
| UniprotID | P35900 (Human),Q9D312 (Mouse) |
| Synonym | Keratin, type I cytoskeletal 20, Cytokeratin-20, CK-20, Keratin-20, K20, Protein IT, KRT20 |
| Note | Centrifuge prior to opening |
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | Assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:200 |
| Immunohistochemistry (IHC) - paraffin | 1:100 (trypsin treatment or microwave treatment recommended) |
| Western Blot (WB) | 1:3,000 |
Excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma).
Reactive polypeptide (specificity): acidic human keratin K20 (Mr 46,000).
Positive control: colon, duodenum, Merkel cells of skin.
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