Goat anti-Human IgG (H&L), F(ab)'2 fragment, HRP conjugated
Katalog-Nummer AS10779
Size : 0.5mg
Marke : Agrisera
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Goat anti-Human IgG (H&L), F(ab)'2 fragment, HRP conjugated
AS10 779 | Clonality: Polyclonal | Host: Goat | Reactivity:Human IgG (H&L), F(ab)'2 fragment
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| Immunogen: | Purified human IgG (H&L) |
| Host: | Goat |
| Clonality: | Polyclonal |
| Purity: | Immunogen affinity purified goat IgG. |
| Format: | Lyophilized |
| Quantity: | 0.5 mg |
| Reconstitution: | For reconstitution add 0,55 ml of sterile water |
| Storage: | Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. |
| Tested applications: | ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB) |
| Recommended dilution: | The optimal working dilution should be determined by the investigator |
| Confirmed reactivity: | Human IgG (H&L), F(ab)'2 fragment |
| Predicted reactivity: | Human IgG (H&L), F(ab)'2 fragment |
| Not reactive in: | No confirmed exceptions from predicted reactivity are currently known |
| Additional information: | HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10% (w/v) BSA, Protease/IgG free 0.1% (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase Purity is ≥90% based on SDS-PAGE. May contain small amounts of intact IgG. |
| Additional information (application): | This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis. No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis. |
| Background: | Goat anti-human IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to HRP which binds to human IgG (H&L), F(ab)'2 fragment in immunological assays. |