Glucose Fluorescent Detection Kit
Katalog-Nummer OKAU00036
Size : 2plate
Marke : Aviva Systems Biology
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Telefonnummer : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for Glucose Fluorescent Detection Kit (OKAU00036) |
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| Application | ELISA-Sandwich | ||||||||||||||||||||||||||||||
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| ELISA Kit Detection Method | Fluorometric | ||||||||||||||||||||||||||||||
| ELISA Kit Linearity | Linearity was determined in human serum samples by taking two diluted samples with known glucose concentrations. One serum sample had a high glucose concentration of 5.77 mg/dL and one had a lower value of 1.86 mg/dL. They were mixed in the ratios given below. The measured concentrations were compared to the expected values based on the ratios used.
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| ELISA Kit Principle | The DetectX® Glucose Fluorescent Detection Kit is designed to quantitatively measure glucose in a variety of samples. Please read the complete kit insert before performing this assay. A ß-D-glucose standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with the Substrate and horseradish peroxidase and the reaction initiated by addition of glucose oxidase. The reaction is incubated at room temperature for 30 minutes. The glucose oxidase reacts with glucose to produce hydrogen peroxide which, in the presence of HRP, converts the substrate into a fluorescent product. The fluorescent product is read at 590 nm with excitation at 570 nm. Increasing levels of glucose cause a linear increase in fluorescence. | ||||||||||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra Assay Precision Three diluted human serum samples were run in replicates of 20 in an assay. The mean and precision of the calculated concentrations were:
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| ELISA Kit Component |
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| Additional Information | Background: Glucose (C6H12O6) is by far the most common carbohydrate. It is a monosaccharide, an aldose, a hexose, and a reducing sugar and is also known as dextrose, because it is dextrorotatory (rotates polarized light clockwise). The structure of glucose is shown below as both the straight chain and cyclic forms. Glucose Structures For all biological and molecular events and for multiple cellular functions, energy is essential. Energy is available in the form of ATP (adenosine triphosphate), most of which is generated through aerobic cellular respiration of carbohydrate and glucose, the major source of biological free energy in higher organisms. Reduced energy levels threaten cellular homeostasis and integrity. Impaired energy metabolism may trigger pro-apoptotic signaling (programmed cell death), oxidative damage, excitotoxicity and impede mitochondrial DNA repair. A serious fall in blood glucose can be characterized by metabolic dysfunction, neuroglycopenia, seizure, and death. A persistent elevation in blood glucose leads to "glucose toxicity." Glucose toxicity contributes to s-cell dysfunction and the pathology grouped together as complications of diabetes. Estrogen-induced signaling pathways in hippocampal and cortical neurons involve the mitochondria to enhance mitochondrial function and to sustain aerobic glycolysis and citric acid cycle oxidative phosphorylation and ATP generation. | ||||||||||||||||||||||||||||||
| :: | Detection Limit: The Limit of Detection was determined as 1.42 ug/dL. Equivalent to 0.00142 mg/dL. | ||||||||||||||||||||||||||||||
| Reconstitution and Storage | 2°C to 8°C | ||||||||||||||||||||||||||||||
| Sample Type | Serum, Plasma, Urine, Buffers and TCM | ||||||||||||||||||||||||||||||
| Sensitivity | Sensitivity was determined as 8.24 ug/dL. Equivalent to 0.00824 mg/dL. |
| Gene Full Name | Glucose |
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