GHRL ELISA Kit (Pig)
Katalog-Nummer OKCD02571
Size : 96Wells
Marke : Aviva Systems Biology
Contact local distributor :
Telefonnummer : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for GHRL ELISA Kit (Pig) (OKCD02571) |
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| Predicted Species Reactivity | Porcine|Sus scrofa | ||||||||||||||||||||||
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| Application | ELISA-Comp | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 2h | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ghrelin (GHRL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ghrelin (GHRL) and unlabeled Ghrelin (GHRL) (Standards or samples) with the pre-coated antibody specific to Ghrelin (GHRL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ghrelin (GHRL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Ghrelin (GHRL) in the sample. | ||||||||||||||||||||||
| ELISA Kit Range | 123.5-10,000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ghrelin (GHRL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ghrelin (GHRL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids | ||||||||||||||||||||||
| Sensitivity | < 52.9 pg/mL | ||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of Ghrelin (GHRL). No significant cross-reactivity or interference between Ghrelin (GHRL) and analogues was observed. | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive Inhibition ELISA |
| Gene Symbol | GHRL |
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| Gene Full Name | ghrelin and obestatin prepropeptide |
| Alias Symbols | appetite-regulating hormone;GH releasing peptide;ghrelin;GRHL;growth hormone secretagogue;growth hormone-releasing peptide;motilin-related peptide;preproghrelin. |
| NCBI Gene Id | 396728 |
| Protein Name | Appetite-regulating hormone |
| Description of Target | Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation (By similarity). |
| Uniprot ID | Q9GKY5 |
| Protein Accession # | NP_998972.1 |
| Nucleotide Accession # | NM_213807.2 |
| Molecular Weight | 118 kDa |