Flow Cytometry: Use 10 µl of neat antibody to label 106 cells in 100 µl.
Reactivity
Equine
Antibody Host
Mouse
Isotype
IgG1
Clonality
Monoclonal
Immunogen
Equine thymocytes. Spleen cells from immunised mice were fused with cells of the X63-Ag 8.653 mouse myeloma cell line.
Specificity
This anitbody CVS4 recognizes Equine CD4, a 58kDa cell surface glycoprotein that is primarily expressed on a subpopulation of equine T lymphocytes and considered to be the equine homologue of human CD4. A study undertaken using Mouse anti Horse CD4, clone CVS4 to identify CD4 on several wild african equid species indicates that the CVS4 clone recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra).
Buffer
Label: PE State: Purified State: Lyophilised purified IgG fraction from Tissue Culture Supernatant Stabilizer: 1% BSA, 5% Sucrose Preservative: 0.09% Sodium Azide Label: R. Phycoerythrin
Reconstitution Method
Restore with 1.0 ml distilled water.
Purification
Affinity Chromatography on Protein G
Conjugation
PE
Storage
Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
