CD34, Recombinant, Human (Endothelial Cell Marker, Cluster designation 34, Hematopoietic progenitor cell antigen CD34, HPCA1)

CD34 is a cell surface antigen formerly known as hemopoietic progenitor cell antigen-1. CD34 is expressed selectively on human hematopoietic progenitor cells. The biological function of CD34 is still unknown. It is used a surface marker for very early hematopoietic stem cells. Cells expressing CD34 respond to SCF (stem cell factor) and LIF in the presence of colony stimulating factors. These factors can induce myeloid differentiation in these cells. The effects of some growth factors on cells expressing CD34 are counteracted by IP-10, a factor the expression of which is induced by IFN-gamma. The proliferation of human CD34 cells is stimulated also by IL-11, and IL-11 synergizes with SCF, IL-3, and GM-CSF. In the presence of human IL-17, fibroblasts can sustain the proliferation of CD34 hematopoietic progenitors and their preferential maturation into neutrophils.||Recombinant CD34, a 385 amino acid long protein, shows a band at 40kD on SDS-PAGE commassie blue stained gel.||Applications:|Suitable for use in ELISA, Inhibition Assays and Western Blot. Other applications not tested. ||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.