The IL-6 receptor system consists of two chains, IL-6R alpha and gp130. The IL-6R alpha is the binding component specific to the IL-6, but does not transduce a signal alone. On the contrary, gp130 transmits signals not only of IL-6, but also IL-6 related cytokines, i.e. LIF, OSM, CNTF, IL-11 and CT-1. The low affinity binding sites for IL-6, is composed of IL-6R alpha alone. At low concentrations, soluble IL-6R alpha acts as an agonist of IL-6 activity.||Applications:|Suitable for use in Flow Cytometry, Immunohistochemistry, FLISA, Immunoprecipitation, Inhibition assays, Functional assays and Immunofluorescence. Other applications not tested.||Recommended Dilutions:|Immunohistochemistry: Frozen sections|Optimal dilutions to be determined by the researcher.||Hybridoma:|Spleen cellls from immunized BALB/c (Iffa Credo) mice were fused with the X63/Ag.8653 mouse myeloma cell line.||Storage and Stability::|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.