Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 106 cells in a suspension.
Reactivity
Human, Rat
Antibody Host
Mouse
Isotype
IgG2a
Clonality
Monoclonal
Immunogen
Recombinant Vaccinia virus containing the human CD105 cDNA
Specificity
The antibody MEM-226 reacts with CD105 (Endoglin), a 180 kDa type I homodimerizing membrane glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.
Buffer
PBS containing 15 mM Sodium Azide and 0.2% (w/v) high-grade protease free BSA as a stabilizing agent Label: FITC State: Liquid purified Ig fraction Label: Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
Conjugation
FITC
Storage
Store undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbR-2 as a receptor for TGFb-1 and TGFb-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFb-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.
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