Calcium (Ca²⁺) Colorimetric Assay Kit

Calcium plays a vital role in the human body, not only as a major component of bones and teeth, but also involved in a series of physiological processes such as muscle contraction, cell adhesion, release of hormones and neurotransmitters, glycogen metabolism, and cell proliferation and differentiation. In plasma, Calcium exists in three forms: free, proteins-bound or complexed with anions. Free Calcium is utilized by the body to maintain physiologic functions. Abnormal calcium may lead to the occurrence of a variety of diseases, so monitoring and maintaining the balance of calcium levels is of great clinical importance for the diagnosis and treatment of related diseases.

The Calcium (Ca²⁺) Colorimetric Assay Kit provides a convenient, highly sensitive, and highly stable method for the detection of calcium ions using o-cresolphthalein complexone (OCPC). Under alkaline conditions, calcium ions can react with OCPC to form a purple complex, which has a maximum absorbance at 575 nm. And the absorbance is proportional to the calcium concentration.

The kit has a wide linear range, maintaining a good linear relationship in the range of 0.2-2 μg of calcium and accurately detecting as little as 0.1 μg of calcium. Liquid samples can be tested directly, while cell and tissue samples can be tested after simple processing.

Figure 1: Calcium standard curve