Biotrend > Asgr1 mouse monoclonal antibody, clone 8D7, FITC
Asgr1 mouse monoclonal antibody, clone 8D7, FITC
Katalog-Nummer AM26170FC-N
Size : 100ug
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Asgr1 Mouse Monoclonal Antibody [Clone ID: 8D7]
| Product Data | |
| Clone Name | 8D7 |
|---|---|
| Application | ELISA, IF, IHC, WB |
| Recommended Dilution | Immunohistochemistry on Frozen Sections (1-3,5): Aceton fixed tissue section were incubated Hybridoma culture supernatant. Flow Cytometry (4). Immunoassay (1,3): Microtiter plates were coated with 100 µl 20 µg/ml for ELISA. Immunfluorescence (7). Western blot (2,4,6): Non-reduced sample treatment and SDS-Page was used. The band size is 42 kDa (4); 10% SDS-PAGE followed by blotting on nitrocellulose or PDFM, block with 10% non-fat dry milk. Recommended Starting Dilutions: 1/50. Positive Control: Rat hepatocytes. Negative Control: Rat kidney, pancreas, small intestine, colon. |
| Reactivity | Human, Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Crude Rat liver membranes extracts. |
| Specificity | This antibody recognizes a subunit-specific epitope on RHL-1 of Rat ASGPR. |
| Buffer | PBS Label: FITC State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 1% BSA Preservative: 0.02% Sodium Azide |
| Concentration | lot specific |
| Purification | Protein G Chromatography |
| Conjugation | FITC |
| Storage | Store undiluted at 2-8°C. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | asialoglycoprotein receptor 1 |
| Database Link | |
| Background | The asialoglycoprotein (ASGP) receptor is a transmembrane hepatocellular surface carbohydrate binding glycoproteins lacking terminal sialic acid residues (asialoglycoproteins). Characterization of the ASGP receptor revealed its functional role in the binding, internalization and transport of a wide range of glycoproteins, which have exposed galactose or N-acetylgalactosamine residues, via the process of receptor-mediated endocytosis (RME). The ASGP receptor can bind a variety of important plasma proteins including transport proteins (i.e. transferrin), enzymes such as alkaline phosphatase, immunoglobulins including IgA, apoptotic hepatocytes, fibronectin and platelets. Additionally, the expression of the ASGP receptor has been clinically correlated to the level of hepatic function that is lost during liver diseases related to cancer, viral hepatitis, and cirrhosis. The ASGP receptor consists of major and minor subunits, which in the rat were identified as rat hepatic lectin (RHL) 1 and RHL 2/3, with molecular weights of respectively 42, 49 and 54 kDa. The selective binding (calcium and pH depended) and uptake of terminal galactosyl bearing proteins requires the formation of hetero-oligomers between these major and minor forms The total ASGP receptor population consisted of two functionally distinct receptor populations, designated State 1 and State 2, which were involved in the endocytosis and intracellular processing of ligands by different pathways. |
| Synonyms | ASGP-R 1, ASGPR 1, Asialoglycoprotein receptor 1, Hepatic lectin H1 |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.