Anti-HLA Class 1 Antigen Bw6 (FITC) Monoclonal Antibody

Applications:|Suitable for use in Flow Cytometry, Cell Typing, Tissue Staining, and Chimerism Studies. Other applications have not been tested.||Recommended Dilution:|Cytotoxicity: Add 0.9ml of 1% BSA in PBS to 100ul of10X working dilution. |Optimal dilutions to be determined by the researcher.||Cytotoxicity:|Expected Results:|Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.|Limitations:|Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at 4°C. Aliquots are stable at 4°C for 12 months after receipt. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.