Anti-Amyloid beta oligomer-specific monoclonal antibody (OMAB), Biotinylated
Katalog-Nummer AS10932B
Size : 50ug
Marke : Agrisera
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Anti-Amyloid beta oligomer-specific monoclonal antibody (OMAB), Biotinylated
AS10 932B | Clonality: Monoclonal | Host: Mouse | Reactivity: amyloid beta
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| Immunogen: | Partly aggregated, recombinant peptide corresponding to the human Abeta (1-40). Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V. |
| Sub class: | IgM |
| Host: | Mouse |
| Clonality: | Monoclonal |
| Purity: | Affinity purified in PBS pH 7.4. |
| Format: | Lyophilized |
| Quantity: | 50 µg |
| Reconstitution: | For reconstitution add 100 µl of sterile water |
| Storage: | Store lyophilized/reconstituted at 4°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Tested applications: | ELISA (ELISA), Immunohistochemistry (IHC) |
| Recommended dilution: | Coating antibody at 2 µg/ml (ELISA), 1 : 500 (IHC) |
| Expected | apparent MW: | 4.5 kDa |
| Confirmed reactivity: | Human Abeta oligomers only |
| Predicted reactivity: | Rat |
| Not reactive in: | No confirmed exceptions from predicted reactivity are currently known |
| Additional information: | OMAB antibody has been purified by by ion-exchange chromatography and is supplied in PBS without any additives as carrier proteins or sodium azide. Binding of OMAB antibody and Abeta oligomers at RT takes about 15 min. |
| Additional information (application): | OMAB antibody is a versatile tool within research of Alzheimer’s disease, A sandwhich ELISA illustrates its potential regarding its high selectivity towards A? oligomers |
| Background: | Soluble oligomeric assemblies of the Amyloid-β peptide are today anticipated to be the direct cause regarding the Alzheimer pathology. As a consequence, oligomeric Aβ-assemblies constitute a very interesting therapeutic target. Identification of Aβ-oligomers is however, technically challenging due to there labile nature and low abundance. Abeta oligomer-specific OMAB antibody is based on the IgM isotype and represents a new concept of Aβ-oligomer binders using a combination of high avidity and very low monovalent affinity. This combination creates a selectivity of the antibody towards the oligomeric fraction and minimizes reactivity towards monomeric species. |
| Selected references: | Richman et al. (2013). In Vitro and Mechanistic Studies of an Anti-Amyloidogenic Self-Assembled Cyclic D,L-#-Peptide Architecture. J. Americal Chemical Societ, Jan 19. Lindhagen-Persson et al. (2010). Amyloid-β Oligomer Specificity Mediated by the IgM Isotype – Implications for a Specific Protective Mechanism Exerted by Endogenous Auto-Antibodies. PLoS ONE. |