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Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

Alkaline Phosphatase (ALP) Activity Assay Kit

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Katalog-Nummer E-BC-K091-S-100

Size : 100Assays

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Detection Principle	Alkaline phosphatase decompose benzene disodium phosphate to produce free phenol and phosphoric acid. Phenol react with 4-aminopyrline in alkaline solution and oxidizes with potassium ferricyanide to form red quinone derivative. The enzyme activity can be calculated indirectly by measuring the OD value.
Synonyms	ALP
Sample Type	Serum,Plasma,Urine,Tissue,Cell
Detection Method	Colorimetric method
Detection Instrument	Spectrophotometer (520 nm)
Research Area	Liver And Renal Function
Other Reagents Required	Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.2 King unit/100 mL
Detection Range	0.2-55.6 King unit/1
Precision	inter-assay CV: 5.6% \| intra-assay CV: 2.1%
Assay Time	1 h

Cat.No.	Product Name	Sizes
E-BC-K094-S	Acid Phosphatase (ACP) Activity Assay Kit	100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	1
Human urine	1
Rat serum	1
Cellular supernatant	1
10% Mouse kidney tissue homogenization	30-50
10% Mouse liver tissue homogenization	1
HePG2 cell	1
10% Mouse brain tissue homogenization	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.