AIF Polyclonal Antibody
Katalog-Nummer bs-0037R-20ul
Size : 20ul
AIF Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
Overview | |
Catalog # | bs-0037R |
Product Name | AIF Polyclonal Antibody |
Applications | WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC |
Reactivity | Human, Mouse, Rat, Pig |
Predicted Reactivity | Dog, Cow, Sheep, Chicken, Rabbit |
Specifications | |
Conjugation | Unconjugated |
Host | Rabbit |
Source | KLH conjugated synthetic peptide derived from human AIF |
Immunogen Range | 131-230/613 |
Clonality | Polyclonal |
Isotype | IgG |
Concentration | 1ug/ul |
Purification | Purified by Protein A. |
Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Storage Condition | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Target | |
Gene ID | 9131 |
Swiss Prot | O95831 |
Subcellular location | Cytoplasm, Nucleus |
Synonyms | AIF; CMT2D; CMTX4; COWCK; NADMR; NAMSD; PDCD8; COXPD6; Apoptosis-inducing factor 1, mitochondrial; Programmed cell death protein 8; AIFM1 |
Background | Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. |
Application Dilution | |
WB | 1:300-5000 |
ELISA | 1:500-1000 |
IHC-P | 1:200-400 |
IHC-F | 1:100-500 |
IF(IHC-P) | 1:50-200 |
IF(IHC-F) | 1:50-200 |
IF(ICC) | 1:50-200 |
ICC | 1:100-500 |