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Acetohydroxamic acid [546-88-3]

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Katalog-Nummer HY-B1235-100mg

Size : 100mg

Contact local distributor :

Telefonnummer : +1 850 650 7790

Marke : MedChemExpress

Contact local distributor :

Telefonnummer : +1 850 650 7790

Acetohydroxamic acid (AHA) is a potent irreversible bacterial and plant urease inhibitor that can be used to study chronic urinary tract infections.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Acetohydroxamic acid Chemische Struktur

CAS. Nr. : 546-88-3

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

Beschreibung

Acetohydroxamic acid (AHA) is a potent irreversible bacterial and plant urease inhibitor that can be used to study chronic urinary tract infections.

Molekulargewicht

75.07

Formel

C₂H₅NO₂

CAS. Nr.

546-88-3

Appearance

Solid

Color

White to off-white

SMILES

CC(NO)=O

Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 250 mg/mL (3330.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (1332.09 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	13.3209 mL	66.6045 mL	133.2090 mL
5 mM	2.6642 mL	13.3209 mL	26.6418 mL
10 mM	1.3321 mL	6.6605 mL	13.3209 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner
Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (27.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (27.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (27.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (1332.09 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Reinheit & Dokumentation

Purity: ≥98.0%

Select Batch:

Data Sheet (271 KB) SDS (418 KB)

COA (246 KB) HNMR (221 KB)

Handling Instructions (2659 KB)

Verweise

[1]. Sharma M, et al. Biotransformation of Acetamide to Acetohydroxamic Acid at Bench Scale Using Acyl Transferase Activity of Amidase of Geobacillus pallidus BTP-5x MTCC 9225. Indian J Microbiol. 2012 Mar;52(1):76-82. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	13.3209 mL	66.6045 mL	133.2090 mL	333.0225 mL
	5 mM	2.6642 mL	13.3209 mL	26.6418 mL	66.6045 mL
	10 mM	1.3321 mL	6.6605 mL	13.3209 mL	33.3023 mL
	15 mM	0.8881 mL	4.4403 mL	8.8806 mL	22.2015 mL
	20 mM	0.6660 mL	3.3302 mL	6.6605 mL	16.6511 mL
	25 mM	0.5328 mL	2.6642 mL	5.3284 mL	13.3209 mL
	30 mM	0.4440 mL	2.2202 mL	4.4403 mL	11.1008 mL
	40 mM	0.3330 mL	1.6651 mL	3.3302 mL	8.3256 mL
	50 mM	0.2664 mL	1.3321 mL	2.6642 mL	6.6605 mL
	60 mM	0.2220 mL	1.1101 mL	2.2202 mL	5.5504 mL
	80 mM	0.1665 mL	0.8326 mL	1.6651 mL	4.1628 mL
	100 mM	0.1332 mL	0.6660 mL	1.3321 mL	3.3302 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Acetohydroxamic acid [546-88-3]

Katalog-Nummer HY-B1235-100mg

Contact local distributor :

Marke : MedChemExpress

Contact local distributor :

Molaritätsrechner

Verdünnungsrechner

Complete Stock Solution Preparation Table

Acetohydroxamic acid Related Classifications

Keywords:

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