Woody plant culture media - Chee & Pool Medium
A woody plant is a plant that produces wood as its structural tissue. Woody plants are usually either trees, shrubs, or lianas. These are usually perennial plants whose stems and larger roots are reinforced with wood produced from secondary xylem.
Chée and Pool medium Contains the macro- and micronutrients as described by Chee and Pool (1987). This medium was developed to improve grape shoot multiplication. This formulation has reduced levels of Chloride, Iodide, and manganese compared to MS. Calcium Chloride was replaced by Calcium Nitrate, thus improving the quality of grape shoots. Substituting calcium nitrate for calcium chloride improved the quality of grapevine shoots produced in culture. Shoot multiplication was dramatically improved by omitting Iodine and lowering the concentration of Manganese. This might be the result of the involvement of both ions in auxin metabolism and transport.
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