Two-Step RT-qPCR mixes - SYBR Green

Two-Step RT-qPCR mixes - SYBR Green


The two-step RT-qPCR involves two distinct reactions, starting with first strand cDNA synthesis (reverse transcription-RT) and then amplifying part of the resulting cDNA by quantitative PCR in a separate tube . Therefore, two-step RT-qPCR is useful for detecting multiple genes in a single RNA sample. The separation of the RT and quantitative PCR reactions makes it possible to optimize the reaction conditions for each step as well as the flexibility with priming by reverse transcription (oligo (dT) primers, random hexamers or gene-specific primers) and PCR DNA polymerase and PCR components). Compared to one-step RT-qPCR, the disadvantages of two-step RT-qPCR include several steps for extended workflow, additional handling and processing of the sample, and increase the risk of contamination and variation results.

two-step-RT-PCR


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Katalog-Nummer
Beschreibung
Cond.
Preis zzgl. MwSt.
AQ201-01
 RTsystem/qPCRsystem: 50rxns×20ul/300rxns×20ul 
AQ301-01
 RTsystem/qPCRsystem: 50rxns×20ul/300rxns×20ul 
AQ202-01
 RTsystem/qPCRsystem: 20rxns×20μl/500rxns×20μl 
IIR-P51-50
 50tests 
IIR-P51-100
 100tests 
MBS402001-5x100
 5x100Reactions 
MBS402086-100
 100Reactions 
MBS402086-5x100
 5x100Reactions 
MBS402001-100
 100Reactions