Zebrafish IL-4 ELISA
Cat# DIY1977Z-003
Size : 1Set
Brand : Kingfisher Biotech
Zebrafish IL-4 ELISA Data
Zebrafish IL-4 ELISA Kit Components
Component | Usage | Quantity | Catalog # |
Anti-Zebrafish IL-4 Polyclonal Antibody | Capture Antibody | 100 µg | KP1975Z-100 |
Biotinylated Anti-Zebrafish IL-4 Polyclonal Antibody | Detection Antibody | 50 µg | KPB1976Z-050 |
Zebrafish IL-4 Recombinant Protein | Standard | 5 µg | RP1034Z-005 |
Zebrafish IL-4 ELISA Specifications
The Zebrafish IL-4 Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Zebrafish IL-4 ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
IL-4 Background
Interleukin-4 (IL-4) induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4. IL-4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is a key regulator in humoral and adaptive immunity. IL-4 induces B-cell class switching to IgE, and up-regulates MHC class II production. Overproduction of IL-4 is associated with allergies.
Alternate Names - IL4, BCGF-1, BCGF1, BSF-1, BSF1, IL-4, interleukin 4