UniGlow™ - One Component Chemiluminescent Substrate
Cat# UniGlow-0100
Size : 100mL
Brand : Rockland Immunochemicals
Contact local distributor :
Phone : +1 850 650 7790
Specifications for UniGlow™ - One Component Chemiluminescent Substrate
Product Details
UniGlow™ - One Component Chemiluminescent Substrate - UniGlow-0020
Western Blot detection, chemiluminescent substrate One Component System, luminol-based chemiluminescent substrate for horseradish peroxidase (HRP) detection
Application Details
ELISA, Microarray, WB - View References
UniGlow™ is a highly sensitive, nonradioactive, enhanced luminol-based, chemiluminescent, ready-to-use peroxidase (HRP) substrate and no mixing is required. UniGlow™- One Component Chemiluminescent Substrate is for use in microwell or membrane applications. Protect from light - UniGlow™ Substrate is a highly sensitive detection reagent allowing for the detection of picograms (6-12 pg) amounts of antigen. Equilibrate to room temperature before use and aliquot into a clean container. Use ~100µL/cm2 of membrane. Optimal detection visualized after contact of substrate with HRP enzyme on membrane for ~1-10min. Blot off excess substrate before imaging. Always carefully optimize all components of individual assays (antigens, antibodies, conjugates…) to minimize background reactivity associated with non-specific binding.
Formulation
1X
Shipping & Handling
Ambient
Store container at 2-8° C prior to opening. Protect from moisture and light. No special shipping conditions or precautions are required.
Expiration date is two (2) years from date of receipt.
Background
Enhanced Chemiluminescence (ECL) involves the enzymatic oxidation of luminol, a process that is catalyzed by peroxidase (HRP) enzyme. In the presence of HRP and a peroxide, luminol is oxidized, and emits light. The intensity of the emitted light is proportional to the amount of HRP-bound target, allowing for the quantitative detection of proteins.
References (6)
Shrestha RL et al. (2021). CENP-A overexpression promotes aneuploidy with karyotypic heterogeneity. J Cell Biol.
Applications
WB, IB, PCA PubMed
Ghosh S et al. (2020). A new microchannel capillary flow assay (MCFA) platform with lyophilized chemiluminescence reagents for a smartphone-based POCT detecting malaria. Nature.
Applications
E, EIA Source
Sthitodhi Ghosh et al. (2020). A new microchannel capillary flow assay (MCFA) platform with lyophilized chemiluminescence reagents for a smartphone-based POCT detecting malaria. Microsyst Nanoeng.
Applications
Microfluidic Point of Care Chip Immunoassay PubMed
Trabbic K., Whalen K., Abarca-Heideman K., Xia L., Temme J., Edmondson E., Gilersleeve J., Barchi J. (2019). A Tumor-Selective Monoclonal Antibody from Immunization with a Tumor-Associated Mucin Glycopeptide. Nature Scientific Reports.
Applications
WB, IB, PCA PubMed
Ohkuni K et al. (2018). N-terminal sumoylation of centromeric histone H3 variant Cse4 regulates its proteolysis to prevent mislocalization to non-centromeric chromatin. G3 (Bethesda).
Applications
WB, IB, PCA PubMed
Shrestha RL et al. (2017). Mislocalization of centromeric histone H3 variant CENP-A contributes to chromosomal instability (CIN) in human cells. Oncotarget.
Applications
WB, IB, PCA PubMed
Related Protocols
Adherent Cell Lysis Protocol
ELISA Protocol
Fluorescent Western Blotting Protocol
Histone Immunoblotting Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Sandwich ELISA Protocol for Collagen
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol
Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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