Size : 500units
Phone : +1 850 650 7790
TransStart® FastPfu DNA Polymerase
Catalog Number： AP221-01
Price：Please inquire first
TransStart® FastPfu DNA Polymerase is a hot start high-fidelity DNA polymerase used for fast PCR. Offering a high amplification efficiency and high extension rate (4 kb/min, it is 8 times that of ordinary Pfu enzymes), TransStart® FastPfu DNA Polymerase amends the defects of low amplification efficiency, low yield and low extension rate (0.5 kb/min) in common Pfu polymerase, and greatly shortens reaction time.
• Offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Blunt-end PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb
• Hot start, high specificity.
• High amplification efficiency.
• Fast and high fidelity.
• Amplifies complex and high GC/ AT templates.
• High fidelity and fast PCR, blunt end cloning, site-directed mutagenesis.
• Amplifies long fragment.
at -20 ℃ for two years
Dry ice (-70 ℃)
| Component | AP221-01/11 | AP221-02/12 | AP221-03/13 |
| TransStart® FastPfu DNA Polymerase | 250 U×1 | 500 U×1 | 500 U×6 |
| 5×TransStart® FastPfu Buffer | 1.2 ml×1 | 1.2 ml ×2 | 1.2 ml ×12 |
| 2.5 mM dNTPs | - / 500 μl×1 | - / 1 ml ×1 | - / 1 ml ×6 |
| 50 mM MgSO4 | 200 μl×1 | 400 μl ×1 | 1 ml ×1 |
| PCR Stimulant | 200 μl×1 | 400 μl×1 | 1 ml ×1 |
| 6×DNA Loading Buffer | 200 μl×1 | 1 ml ×1 | 1 ml ×2 |
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