Transferrin (N-term) Mouse Monoclonal Antibody [Clone ID: HTF-14]

Specifications

Product Data
Clone Name	HTF-14
Applications	ELISA, FN, IF, IHC, R, WB
Recommended Dilution	Western blot: non-reducing conditions. Immunohistochemistry on paraffin sections: 10 µg/ml. Positive control: placenta. Immunocytochemistry. ELISA. RIA. Functional application: blocks binding of transferrin to the receptor.
Reactivities	Human, Porcine, Rabbit
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Purified porcine transferrin
Specificity	This antibody recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.
Formulation	Phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized State: Azide Free State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Purified by protein-A affinity chromatography.
Conjugation	Unconjugated
Storage	Store undiluted at 2-8°C. DO NOT FREEZE!
Stability	Shelf life: one year from despatch.
Database Link	UniProt ID P09571
Background	Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.
Synonyms	Serotransferrin, Siderophilin
Reference Data