Reagents and instruments for immunology, cell biology and molecular biology.
 
> Total Iron Binding Capacity (TIBC) and Serum Iron Assay Kit

Total Iron Binding Capacity (TIBC) and Serum Iron Assay Kit

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Cat# E-BC-K758-M-48T

Size : 48T

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Detection Principle The excess iron is added to the serum to bind all the transferrin in the serum, and the excess iron is adsorbed by adding the iron adsorbent. The iron bind with the transferrin is separated from the protein by the action of acid solution and reductant. Fe3+ in serum is reduced to Fe2+, Fe2+ binds with bipyridine to form pink complex. In a certain range, the amount of iron ions is positively correlated with the color, and the measured iron content is TIBC. Subtracting serum iron value from TIBC is called unsaturated iron binding capacity (UIBC).
Sample Type Serum,Plasma
Detection Method Colorimetric method
Detection Instrument Microplate reader (510-530 nm, optimum wavelength: 520 nm)
Research Area Metabolic Diseases ,  Liver And Renal Function ,  Inorganic Ions
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.14 mg/L TIBC
Detection Range 0.31-50 mg/L TIBC
Precision inter-assay CV: 1.5 % | intra-assay CV: 2.3 %
Assay Time 40 min
Cat.No. Product Name Sizes
E-BC-K772-M Total Iron Colorimetric Assay Kit 96T , 48T
E-BC-K773-M Ferrous Iron Colorimetric Assay Kit 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human serum 1
Rat serum 1
Porcine serum 1
Rabbit serum 1
Chicken serum 1
Machin serum 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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