K1094-5000 | T5 Exonuclease (RNase-free) Biotrend

Description

T5 Exonuclease is a nucleic acid exonuclease that degrades double- or single-stranded DNA in a 5' →3' direction. Degradation can begin either from the 5' end of double- or single-stranded DNA, or from gaps or nicks in linear or cyclic double-stranded DNA, but not from superhelix double-stranded DNA. T5 Exonuclease is often used for Gibson Assembly; Degradation of linear single-stranded, double-stranded DNA or absent plasmid DNA; Removal of incomplete ligation products from the linked cyclic double-stranded DNA; Degradation of linear and absent plasmid DNA to obtain high-purity supercoiled plasmid DNA; Removal of metamorphic DNA produced during plasmid extraction by alkali cleavage; Improve transfection efficiency of small extract plasmid cDNA libraries.

Quality Control

Protocol

Related Biological Data

Components and Storage

Components	1000 U	5000 U	10000 U
T5 Exonuclease (RNase-free)	0.1 ml	0.5 ml	1 ml
10X T5 Exonuclease Reaction Buffer	1 ml	1 ml x5	1ml x10
Store at -20 °C.

T5 Exonuclease (RNase-free)

Cat# K1094-5000

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Brand : APExBIO Technology

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Description

Quality Control

Related Biological Data

Components and Storage

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