Specifications

Product Data
Clone Name	STRO-1
Applications	FC, IF
Recommended Dilution	Flow Cytometry. Immunocytochemistry.
Reactivities	Human
Host	Mouse
Isotype	IgM
Clonality	Monoclonal
Immunogen	Human CD34 positive bone marrow cells.
Specificity	This antibody recognizes the cell surface antigen STRO1 expressed by bone marrow mesenchymal stromal cells and nucleated erythroid precursors, but not by committed hematopoietic progenitors.
Formulation	Tris buffered saline (TBS), pH~8.0 State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 15mM Sodium Azide
Concentration	lot specific
Purification	Precipitation Methods and Ion Exchange Chromatography
Conjugation	Unconjugated
Storage	Store undiluted at 2-8°C. DO NOT FREEZE!
Stability	Shelf life: one year from despatch.
Background	STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO-1. Approximately 10% of mononuclear cells, greater than 95% of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.
Reference Data