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> Sodium citrate (dihydrate) [6132-04-3]

Sodium citrate (dihydrate) [6132-04-3]

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Cat# HY-B1610-500mg

Size : 500mg

Brand : MedChemExpress

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Sodium citrate dehydrate (Trisodium citrate dihydrate) is a natural product with oral activity that can be found in citrus fruits. Sodium citrate dehydrate can inhibit the proliferation of tumor cells and induce apoptosis. Sodium citrate dehydrate has antibacterial, anti-tumor and antioxidant activities. Sodium citrate dehydrate can be prepared as a cosolvent or buffer.

For research use only. We do not sell to patients.

Sodium citrate dihydrate Chemical Structure

Sodium citrate dihydrate Chemical Structure

CAS No. : 6132-04-3

This product is a controlled substance and not for sale in your territory.

Based on 3 publication(s) in Google Scholar

Other Forms of Sodium citrate dihydrate:

  • Lithium citrate tetrahydrate In-stock
  • Sodium citrate dihydrate, meets USP testing specifications In-stock
  • Citric acid trisodium In-stock
  • Citric acid In-stock
  • Citric acid tripotassium hydrate In-stock
  • Sodium citrate dihydrate, United States Pharmacopeia (USP) Reference Standard Get quote
  • Sodium citrate dihydrate, for molecular biology Get quote
Description

Sodium citrate dehydrate (Trisodium citrate dihydrate) is a natural product with oral activity that can be found in citrus fruits. Sodium citrate dehydrate can inhibit the proliferation of tumor cells and induce apoptosis. Sodium citrate dehydrate has antibacterial, anti-tumor and antioxidant activities. Sodium citrate dehydrate can be prepared as a cosolvent or buffer[1][2][3].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Sodium citrate dehydrate (100 μM) can enhance the inhibition effect of rosemary acid (RA) on Vibrio bacterial[1].
Sodium citrate dehydrate (5-20 mM, 24-48 h) induces apoptosis of human gastric cancer cell line MGC-803 by inhibiting glycolysis and promoting mitochondria-regulated apoptosis pathway[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Sodium citrate dihydrate Related Antibodies

Cell Viability Assay[2]

Cell Line: MGC-803
Concentration: 1.25, 2.5, 5, 10,15, 20, 30, 40, 60 mM
Incubation Time: 24, 48 h
Result: Decreased cell viability in a dose-and time-dependent manner.
The IC50 value is 10.08 mM after 48 h.

Apoptosis Analysis[2]

Cell Line: MGC-803
Concentration: 5, 10, 20 mM
Incubation Time: 24 h
Result: Decreased the mRNA and protein expressions of anti-apoptotic Bcl-2 and Survivin.
Increased the mRNA and protein expression level of proapoptotic Bax, caspase-3 and Cyt-c.

Western Blot Analysis[2]

Cell Line: MGC-803
Concentration: 5, 10, 20 mM
Incubation Time: 24, 48 h
Result: Induced cell apoptosis in a dose-and time-dependent manner.
Increased the G2/M phase arrest.
In Vivo

Sodium citrate dehydrate (216, 746 mg/kg, gavage for 16 weeks) can inhibit endoplasmic reticulum stress in rats with chronic renal failure induced by adenine (HY-B0152)[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adenine-Induced Chronic Renal Failure Rat Model[3]
Dosage: 216, 746 mg/kg
Administration: i.g.
Result: Decreased the expression of renal fibrosis-related proteins TGF-β1 and CIV.
Decreased the expression of PERK, ATF6 and CHOP.
Clinical Trial
Molecular Weight

294.10

Formula

C6H9Na3O9
CAS No.

6132-04-3
Appearance

Solid

Color

White to off-white

SMILES

O=C(CC(C(O[Na])=O)(O)CC(O[Na])=O)O[Na].O.O
Structure Classification
  • Ketones, Aldehydes, Acids
Initial Source
  • Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 125 mg/mL (425.03 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4002 mL 17.0010 mL 34.0020 mL
5 mM 0.6800 mL 3.4002 mL 6.8004 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (340.02 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

  • [1]. Lu P, et al. Rosmarinic Acid and Sodium Citrate Have a Synergistic Bacteriostatic Effect against Vibrio Species by Inhibiting Iron Uptake. Int J Mol Sci. 2021 Dec 1;22(23):13010.  [Content Brief]

    [2]. Guo X,et al. 3-Bromopyruvate and sodium citrate induce apoptosis in human gastric cancer cell line MGC-803 by inhibiting glycolysis and promoting mitochondria-regulated apoptosis pathway. Biochem Biophys Res Commun. 2016 Jun 17;475(1):37-43.  [Content Brief]

    [3]. Ou Y, et al. Sodium Citrate Inhibits Endoplasmic Reticulum Stress in Rats with Adenine-Induced Chronic Renal Failure. Am J Nephrol. 2015;42(1):14-21.  [Content Brief]

  • [1]. Lu P, et al. Rosmarinic Acid and Sodium Citrate Have a Synergistic Bacteriostatic Effect against Vibrio Species by Inhibiting Iron Uptake. Int J Mol Sci. 2021 Dec 1;22(23):13010.

    [2]. Guo X,et al. 3-Bromopyruvate and sodium citrate induce apoptosis in human gastric cancer cell line MGC-803 by inhibiting glycolysis and promoting mitochondria-regulated apoptosis pathway. Biochem Biophys Res Commun. 2016 Jun 17;475(1):37-43.

    [3]. Ou Y, et al. Sodium Citrate Inhibits Endoplasmic Reticulum Stress in Rats with Adenine-Induced Chronic Renal Failure. Am J Nephrol. 2015;42(1):14-21.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 3.4002 mL 17.0010 mL 34.0020 mL 85.0051 mL
5 mM 0.6800 mL 3.4002 mL 6.8004 mL 17.0010 mL
10 mM 0.3400 mL 1.7001 mL 3.4002 mL 8.5005 mL
15 mM 0.2267 mL 1.1334 mL 2.2668 mL 5.6670 mL
20 mM 0.1700 mL 0.8501 mL 1.7001 mL 4.2503 mL
25 mM 0.1360 mL 0.6800 mL 1.3601 mL 3.4002 mL
30 mM 0.1133 mL 0.5667 mL 1.1334 mL 2.8335 mL
40 mM 0.0850 mL 0.4250 mL 0.8501 mL 2.1251 mL
50 mM 0.0680 mL 0.3400 mL 0.6800 mL 1.7001 mL
60 mM 0.0567 mL 0.2834 mL 0.5667 mL 1.4168 mL
80 mM 0.0425 mL 0.2125 mL 0.4250 mL 1.0626 mL
100 mM 0.0340 mL 0.1700 mL 0.3400 mL 0.8501 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Sodium citrate dihydrate Related Classifications

  • Anti-infection Metabolic Enzyme/Protease Apoptosis
  • Bacterial Endogenous Metabolite Apoptosis
Help & FAQs

Keywords:

Sodium citrate dihydrate6132-04-3Trisodium citrate dihydrateCitric acid trisodium salt dihydrateBacterialEndogenous MetaboliteApoptosisAntibacterialAntitumorMGC-803AdenineAntioxidantInhibitorinhibitorinhibit

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