SARS-CoV-2 (COVID-19) Spike (cleavage site)

Cat# 583279-100ug

Size : 100ug

Brand : US Biological

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583279 SARS-CoV-2 (COVID-19) Spike (cleavage site)

Clone Type
Polyclonal
Host
rabbit
Isotype
IgG
Grade
Affinity Purified
Applications
E IF WB
Accession #
QHD43416
Shipping Temp
Blue Ice
Storage Temp
-20°C

Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a Spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. Coronavirus invades cells through Spike (S) glycoproteins, a class I fusion protein. It is the major viral surface protein that coronavirus uses to bind to the human cell surface receptor. It also mediates the fusion of host and viral cell membrane, allowing the virus to enter human cells and begin infection (3). The spike protein is the major target for neutralizing antibodies and vaccine development (4). The protein modeling suggests that there is strong interaction between Spike protein receptor-binding domain and its host receptor angiotensin-converting enzyme 2 (ACE2), which regulate both the cross-species and human-to-human transmissions of COVID-19 (5). The recent study has shown that the SARS-CoV-2 spike protein binds ACE2 with higher affinity than SARS-CoV spike protein (6).||Applications: |Suitable for use in ELISA, Immunofluorescence and Western Blot. Other applications not tested.||Recommended Dilution:|Immunofluorescence: 20ug/ml|Western Blot: 0.5-4ug/ml|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Concentration: ~1mg/ml|Form: Supplied as a liquid in PBS, 0.02% sodium azide.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Synthetic peptide corresponding to 12 amino acids near the center of SARS-CoV-2 (COVID-19) Spike glycoprotein.|Specificity: Recognizes SARS-CoV-2 (COVID-19) Spike. Cross-reactivity: SARS-CoV Spike proteins: (25%)||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Synthetic peptide corresponding to 12 amino acids near the center of SARS-CoV-2 (COVID-19) Spike glycoprotein.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes SARS-CoV-2 (COVID-19) Spike. Cross-reactivity: SARS-CoV Spike proteins: (25%)
References
1. Gorbalenya. bioRxiv: 2020.|2. Hui et al. Int J Infect Dis. 2020;91:264-266.|3. Belouzard et al. Viruses. 2012;4(6):1011-33.|4. Lee et al. J Virol. 2006;80(8):4079-87.|5. Wan et al. J Virol. 2020.|6. Wrapp et al. Science. 2020.