SAP130 ELISA Kit (Chicken)
Cat# OKWB00132
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for SAP130 ELISA Kit (Chicken) (OKWB00132) |
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| Predicted Species Reactivity | Chicken|Gallus gallus | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | Aviva Systems Biology Sap130 ELISA Kit (Chicken) (OKWB00132) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with Sap130. Sample or standards are added to the wells along with a Sap130 biotinylated detector antibody and incubated. The Sap130 found in the sample or standards inhibits the Sap130 detector antibody from binding to the immobilized Sap130 on the plate. Excess unbound sample or standard Sap130 and detector antibody is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of Sap130 biotinylated detector antibody bound in the well and inversely proportional to the amount of Sap130 which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 31.25-2000pg/ml | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Mean Intra-Assay Precision <10% Mean Inter-Assay Precision <12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 18.75 pg/mL | ||||||||||||||||||||||
| Assay Info | Assay Type: Quantitative Competitive Inhibition ELISA |
| Gene Symbol | SAP130 |
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| Gene Full Name | Sin3A associated protein 130 |
| Alias Symbols | 130 kDa Sin3-associated polypeptide;histone deacetylase complex subunit SAP130;Sin3A associated protein 130kDa;sin3-associated polypeptide p130. |
| NCBI Gene Id | 424756 |
| Protein Name | Histone deacetylase complex subunit SAP130 |
| Description of Target | Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes (By similarity). |
| Uniprot ID | Q5F3U0 |
| Protein Accession # | NP_001026472 |
| Nucleotide Accession # | NM_001031301 |