Aviva Systems Biology RuBisCO ELISA Kit (Plant) (OKCA00374) is based on a competitive enzyme immuno assay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for RuBisCO and Horseradish Peroxidase (HRP) conjugated RuBisCO. The competitive inhibition reaction is launched between with HRP labeled RuBisCO and unlabeled RuBisCO with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of RuBisCO in the sample. The color development is stopped and the intensity of the color is measured.
ELISA Kit Range
3.13 ug/mL - 800 ug/mL
ELISA Kit Recovery
Sample 1
Sample Type
Average Recovery
Range
leaf tissue (n=5)
0.95
89-103%
ELISA Kit Reproducibility
Mean Intra-Assay Precision %CV: < 10% (n = 20)
Mean Inter-Assay Precision %CV: < 20% (n = 20)
ELISA Kit Component
Component
Amount
Anti-RuBisCO Microplate
96 Well (12 x 8 Well Strips)
RuBisCO Standard
2 x 500 uL
100X Antibody
1 x 60 uL
100X Anti-RuBisCO Antibody HRP Conjugate
1 x 120 uL
Antibody Diluent
1 x 10 mL
25X HRP-Conjugate Diluent
1 x 20 mL
Sample Extraction Buffer
1 x 20 mL
Sample Diluent
2 x 20 mL
25X Wash Buffer
1 x 20 mL
TMB Substrate
1 x 10 mL
Stop Solution
1 x 10 mL
Additional Information
CAS#: 9027-23-0
Reconstitution and Storage
Store as indicated in product manual.
Sample Type
Plant tissues
Sensitivity
< 3.13 ug/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of plant RuBisCO. No significant cross-reactivity or interference between plant RuBisCO and analogues was observed.