Renilla Luciferase Assay Kit 2.0, 150 Assays
Cat# 30082-1
Size : 1kit
Brand : Biotium
Contact local distributor :
Phone : +1 850 650 7790
Product Description
This kit is designed to measure Renilla luciferase (RLuc) activity in transfected cell reporter gene assays.
Features
- Highly sensitive & linear flash-type RLuc assay
- Easy-to-handle water-soluble substrates
- Excellent performance & great value compared to other suppliers
Kit Components
- 5X Passive Lysis Buffer
- Renilla Luciferase Assay Buffer 2.0
- Aquaphile™ Coelenterazine
Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. It commonly is used in multiplex transcriptional reporter assays or as a normalizing transfection control for firefly luciferase assays. Renilla luciferase, a monomeric 36,000 Dalton protein, catalyzes coelenterazine oxidation by oxygen to produce light. The enzyme does not require post-translational modification for its activity and may function as a genetic reporter immediately following translation. Coelenterazine also emits light from enzyme-independent oxidation, a process known as autoluminescence. The autoluminescence is enhanced by superoxide anion and peroxynitrite in cells and tissues. This assay kit utilizes a special buffer formulation designed to yield reliable, linear measurements of Renilla luciferase activity with minimal autoluminescence background and superior sensitivity.
Note: This kit is a flash-type luminescence assay, which requires luminescence to be measured immediately after adding reagents to your sample. It requires a single-tube luminometer or a luminescence microplate reader with reagent injectors.
Easy-to-Use for Consistent Results
This kit is a new and improved replacement for the Renilla Luciferase Assay Kit (catalog no. 30004). In addition to providing a simplified protocol, the Renilla Luciferase Assay Kit 2.0 features Biotium’s Aquaphile™ Coelenterazine, a water soluble substrate that can be stored at -20°C with minimal evaporation, unlike methanol solutions of coelenterazine.
The stated number of assays is based on 96-well assay format. The kits include enough Passive Lysis Buffer to perform the stated number of assays in plate sizes ranging from 96-well to 24-well. If needed, additional 5X Passive Lysis Buffer (catalog no. 99912) can be purchased separately (see below).
Biotium also offers the Firefly & Renilla Luciferase Single Tube Assay Kit (catalog no. 30081), a combined luciferase assay kit for sequential measurement of Firefly and Renilla luciferase activity sequentially in the same sample with high sensitivity and linearity.
More Luciferase Kits & Related Products
| Biotium Cat. No. | Product | Description | Promega Assay | Promega Cat. No. |
|---|---|---|---|---|
| 30138 | Steady-ATP™ HTS Viability Assay Kit | Highly sensitive ATP-based cell luminescent viability assay with signal half-life of 5 hours for high-throughput screening in multi-well plates. | CellTiter-Glo® Luminescent Cell Viability Assay | G7570, G7571, G7572, G7573 |
| 30085 | Firefly Luciferase Assay Kit 2.0 | Flash-type firefly luciferase assay kit. | Firefly Luciferase Assay System | E1483, E1500, E1501, E4030, E4530, E4550 |
| 30075 | Firefly Luciferase Assay Kit (Lyophilized) | Firefly luciferase assay kit with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C. | ||
| 30028 | Steady-Luc™ Firefly HTS Assay Kit | Glow-type firefly luciferase assay with signal half-life of about 3 hours for high-throughput screening in multi-well plates. | Steady-Glo® Assay System | E2510, E2520, E2550 |
| 30028L | Steady-Luc Firefly HTS Assay Kit (Lyophilized) | Steady-Luc kit with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C. | ||
| 30082 | Renilla Luciferase Assay Kit 2.0 | Flash-type Renilla luciferase assay kit. | Renilla Luciferase Assay System | E2810, E2820 |
| 30081 | Firefly and Renilla Luciferase Single Tube Assay Kit | Assay kit for measuring firefly and Renilla activities sequentially in the same sample in a single tube. | Dual-Luciferase® Assay System (Firefly & Renilla) | E1910, E1960, E1980 |
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