Recombinant Rat GM-CSF

Cat# 300-03-1MG

Size : 1mg

Brand : FUJIFILM

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Recombinant Rat GM-CSF

Details

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is hematopoietic growth factor produced by endothelial cells, monocytes, fibroblasts, and T cells. GM-CSF stimulates the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells. GM-CSF promotes immune system development and regulates neutrophil function during infection.

Instructions

Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80 ºC and avoid repeat freeze thaws.   

Technical Specifications

Alternate Names CSF-2, Pluripoietin-α, MGI1GM
Accession Number P48750
Source Genetically modified E.coli
Predicted Molecular Mass Monomer, 14.7 kDa (128 aa)
AA Sequence MAPTRSPNPV TRPWKHVDAI KEALSLLNDM RALENEKNED VDIISNEFSI QRPTCVQTRL KLYKQGLRGN LTKLNGALTM IASHYQTNCP PTPETDCEIE VTTFEDFIKN LKGFLFDIPF DCWKPVQK
Formulation Lyophilized from a 0.2 micron filtered solution containing 20 mM sodium bicarbonate, pH 8.5
Product Reconstitution Sterile water at 0.1 mg/mL
Country of Origin USA

 

Specification* Method of Determination Acceptance Criteria
Purity Reducing and Non-Reducing SDS PAGE ≥ 95%
Endotoxin Kinetic LAL ≤ 0.1 EU/µg
Biological Activity (ED50) FDC-P1 cell proliferation ≤ 0.02 ng/mL

 

*Lot-specific values for the above specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements.

Specifications

Storage
At or below -20°C
Shelf Life
12 months from date of receipt when stored at -20 to -80 ºC as supplied.