Recombinant Protein Human TNF RI - Fc Chimera

Symansis' purified TNF RI - Fc Chimera^hcx has N-linked and probably O-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F and a further slight drop in observed MW on SDS-PAGE after treatment with a glycosidase cocktail. Symansis' TNF RI - Fc Chimera^hcx consists of 5-20% carbohydrate by weight.

Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

1D SDS-PAGE of Symansis' hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation.

1. Chen G & Goeddel DV (2002) Science 296: 1634-5.