Recombinant Protein Human M-CSF R Fc Chimera

Symansis' M-CSF R - Fc^hcx Chimera has N-linked and O-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F and a further drop after treatement with a glycosidase cocktail. Symansis' M-CSF R - Fc^hcx Chimera contains 20-45% carbohydrate by weight.

Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

1D SDS-PAGE of Symansis' hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation.

1. Bourette and Rohrschneider (2000) Growth Factors 17:155-166