Rat Ig, Mouse ads Antibody : Biotin
Cat# OASB01900
Size : 1.0mg
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
Goat Anti-Rat Ig Antibody - Biotin Conjugated (OASB01900)
| Datasheets/Manuals | Printable datasheet for Goat Anti-Rat Ig Antibody - Biotin Conjugated (OASB01900) |
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| Tested Species Reactivity | Rat |
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| Predicted Species Reactivity | Rat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Conjugation | Biotin |
| Application | FC, IHC-F, IHC-P, ICC, WB |
| Additional Information | Description: Pooled antisera from goats hyperimmunized with rat IgG and IgM; purified by affinity chromatography on pooled rat Ig covalently linked to agarose; reacts with the heavy and light chains of rat IgG and IgM; cross-adsorbed against mouse immunoglobulins and pooled sera for minimal reactivity |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Rat IgG and IgM |
| Purification | Affinity chromatography on pooled rat IgM + IgG covalently linked to agarose |
| Concentration | 0.5 mg/mL |
| Specificity | Ig |
| Characterization | To ensure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry forconformance to characteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Immunofluorescence: FLISA: ELISA: FITC and BIOT conjugates R-PE and APC conjugates TRITC and TXRD conjugates AP conjugate HRP conjugate BIOT conjugate <= 1 ug/106 cells <= 0.1 ug/106 cells 1:100-1:400 1:2,000-1:4,000 1:4,000-1:8,000 1:5,000-1:20,000 |
| Application Info | Direct and indirect immunofluorescent staining, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Immunoblotting, Immunohistochemistry |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC), rhodamine (TRITC), and Texas Red (TXRD) conjugates are supplied as 1.0 mgin 1.0 mL PBS/NaN3. Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 0.5 mg in 1.0 mL or0.25 mg in 0.5 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed. |
| Cross Absorption | Pooled mouse sera and purified mouse paraproteins |
| Gene Full Name | B-cell antigen receptor complex-associated protein alpha chain |
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