Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivity
Rabbit
Antibody Host
Goat
Clonality
Polyclonal
Immunogen
Rabbit IgG whole molecule
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rhodamine and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: TRITC State: Lyophilized Fab fragments Label: Tetramethylrhodamine isothiocyanante ; Molecular Weight 444 daltons) Absorption emission: 550 nm / 570 nm Molar radio: 2.0 moles TRITC per mole of Goat IgG Fab
Reconstitution Method
Restore with 1.0 ml of deionized water or equivalent.
Concentration
lot specific
Purification
Immunoaffinity chromatography
Conjugation
TRITC
Storage
Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
