Primary Human Glomerular Mesangial Cells (ACBRI 127)

Increase Biological Relevance with Human Primary Cells

Our antibody-free primary cells can offer a more biologically relevant cell culture tool for scientists to enhance their research insights. These primary cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500) and subsequently grown and passaged in Cell Systems Complete Classic Medium (4Z0-500). The cells are cryopreserved in Cell Systems Cell Freezing Medium (4Z0-705).

  • Isolated from normal, healthy donor tissue
  • Available at Passage 3 (<12 cumulative population doublings)
  • Each vial contains approximately 1 x 106 cells
  • Each vial will initiate a Passage 4 cell culture in a 75 cm2 flask
  • Available in reserved lots to enhance consistency in your research program

Each vial of cells is shipped to customers with 1mL of Bac-Off® and .25mL of CultureBoost™.

A Selection of Citations for ACBRI 377 from Scientific Journals
Discover additional research on Google Scholar that utilizes Cell Systems primary cells.

 

Standard Tests

TESTS RESULTS

HIV Serologic Test (donor level HIV AB EIA)

Negative            

HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab)

Negative

Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level)

Negative

Retail Production (P3)Tests

TESTS RESULTS

Bacterial Sterility (culture method) by independent lab

Pass

Fungal Sterility (culture method) by independent lab

Pass

Mycoplasma Sterility (culture method) by independent lab

Pass

Cell Markers and Functional Tests

TESTS RESULT

Inducible expression of CD 62E (E-Selectin)

> 90% positive by immunofluorescence

Cytoplasmic VWF / Factor VIII

> 95% positive by immunofluorescence

Cytoplasmic uptake of Di-I-Ac-LDL

> 95% positive by immunofluorescence

COMPANION PRODUCTS:

OPTIMIZE YOUR RESEARCH
WITH HUMAN PRIMARY CELLS

Avoid the obstacles from immortalized cell lines or animal models and gain more pertinent insights into human cell biology.

Cell Systems cells are available for in vitro research purposes only and may not be transferred out of the direct control of the recipient Institution/Agency/Organization. Cell Systems cells may not be genetically altered in any way without prior written permission from Cell Systems. Use of Cell Systems materials (evidenced by placement of any order for product) constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient Institution/Agency/Organization.

Cell Systems was created to further the knowledge of eukaryotic cell biology through laboratory research, publications and teaching. Cell Systems provides cells and cell culture products to other research entities - public and private.