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> Phosphotyrosine (P-Tyr)(PY265), 0.2mg/mL

Phosphotyrosine (P-Tyr)(PY265), 0.2mg/mL

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Cat# BNUB0265-500

Size : 500uL

Brand : Biotium

Contact local distributor :


Phone : +1 850 650 7790

Phosphotyrosine Monoclonal Mouse Antibody (PY265)

Product Attributes

Antibody number

#0265
Antibody reactivity (target)

Phosphotyrosine
Antibody type

Primary
Host species

Mouse
Clonality

Monoclonal
Clone

PY265
Isotype

IgG1, kappa
Molecular weight

Depends upon the phosphorylated target
Synonyms

P-Tyr
Entrez gene ID

Not Applicable
SwissProt

Not Applicable
Unigene

Not Applicable
Immunogen

Phosphotyrosine conjugated to BSA
Species reactivity

All species
Antibody application notes

For coating for ELISA, order Ab without BSA, Optimal dilution and staining procedure for a specific application should be determined by user, Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
Positive control

MCF-7, MDA-231, T47-D cells. Breast carcinoma.
Shipping condition

Room temperature
Storage Conditions

Note: store BSA-free antibodies at -10 to -35°C, Store at 2 to 8 °C, Protect fluorescent conjugates from light
Regulatory status

For research use only (RUO)
Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended
Product origin

Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.

Technology

CF® Dyes
Considerations for Immunofluorescence Staining
Primary & Secondary Antibody Conjugates
Download Datasheet

Product Description

Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email info@biotrend.com to inquire about stock status and lead times before placing your order.

Catalog Number Key for Primary Antibody Conjugates

Antibody # prefix Conjugation Ex/Em (nm) Laser line Detection channel Dye Features
BNC04 CF®405S 404/431 405 DAPI (microscopy), AF405 CF®405S Features
BNC88 CF®488A 490/515 488 GFP, FITC CF®488A Features
BNC68 CF®568 562/583 532, 561 RFP, TRITC CF®568 Features
BNC94 CF®594 593/614 561 Texas Red® CF®594 Features
BNC40 CF®640R 642/662 633-640 Cy®5 CF®640R Features
BNC47 CF®647 650/665 633-640 Cy®5 CF®647 Features
BNCB Biotin N/A N/A N/A
BNUB Purified N/A N/A N/A
BNUM Purified,
BSA-free		 N/A N/A N/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.

Note: Listed references are for this antibody clone sold by Biotium and other suppliers.

 

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