Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells in 100 µl.
Reactivity
Rat
Antibody Host
Mouse
Isotype
IgG1
Clonality
Monoclonal
Immunogen
Activated, Lewis rat derived microglial cells. Spleen cells from immunised BALB/c mouse were fused with cells of the mouse SP2 myeloma cell line.
Specificity
This antibody recognises PECAM-1 (CD31), a type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes. Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen. (3) We recommend SM272LE for use in functional studies.
Buffer
PBS, pH 7.4 containing 1% BSA as stabilizer and 0.09% Sodium Azide as preservative. Label: FITC State: Liquid purified IgG fraction Label: Fluorescein Isothiocyanate Isomer I
Concentration
lot specific
Purification
Affinity Chromatography on Protein G
Conjugation
FITC
Storage
Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets. CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).
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