Flow Cytometry Use 10 ml of neat-1/10 diluted antibody to label 100 µl of whole blood or 10e6 cells in 100 µl.
Reactivity
Human, Porcine
Antibody Host
Mouse
Isotype
IgG1
Clonality
Monoclonal
Immunogen
Porcine CD31/human IgGFc fusion protein.
Specificity
This anitibody recognises CD31, a 140 kD cell surface protein also called PECAM-1, expressed by various cell types, but particularly by endothelial cells. Clone LCI-4 has been shown to stimulate tyrosine phosphorylation of CD31 on endothelial cells.
Buffer
PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer. Label: APC State: Lyophilized purified IgG fraction. Label: Allophycocyanin
Reconstitution Method
Restore with 1.0 ml distilled water.
Concentration
lot specific
Purification
Affinity Chromatography on Protein G.
Conjugation
APC
Storage
Store the antibody undiluted Prior to and After reconstitution at 2-8°C. DO NOT FREEZE!. This product is photosensitive and should be protected from light.
Cell adhesion molecules are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth and are thought to play an important role in embryogenesis and development. Neuronal cell adhesion molecule (NCAM) expression is observed in a variety of human tumors including neuroblastomas, rhabdomyosarcomas, Wilms’ tumors, Ewing’s sarcomas and some primitive myeloid malignancies. The intracellular adhesion molecule-1 (ICAM-1), also referred to as CD54, is an integral membrane protein of the immunoglobulin superfamily and recognizes the B2α1 and B2αM Integrins. PECAM-1 (platelet/endothelial cell adhesion molecule-1), also referred to as CD31, is a glycoprotein expressed on the cell surfaces of monocytes, neutrophils, platelets and a subpopulation of T cells. VCAM-1 (vascular cell adhesion molecule-1) was first identified as an adhesion molecule induced on human endothelial cells by inflammatory cytokines such as IL-1, tumor necrosis factor (TNF) and lipopolysaccharide (LPS). The KALIG gene encodes a nerve cell adhesion molecule (NCAM)-like protein and is deleted in 66% of patients with Kallmann’s syndrome, anosmia with secondary hypogonadism.
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