Reagents and instruments for immunology, cell biology and molecular biology.
 
> OT (Oxytocin) ELISA Kit

OT (Oxytocin) ELISA Kit

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Cat# E-EL-0029-96T

Size : 96T

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Product Summary
Sensitivity 9.38 pg/mL
Detection Range 15.63-1000 pg/mL
Sample Volume 50 μL
Total Assay Time 2 h 30 min
Reacitivity Universal
Specificity This kit recognizes Universal OT in samples.No significant cross-reactivity or interference between Universal OT and analogues was observed
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Competitive
Assay Type Competitive-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal OT. During the reaction, Universal OT in the sample or standard competes with a fixed amount of Universal OT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal OT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal OT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Uniport ID H:P01178 M:P35454 R:P01179
Research Area Neuroscience , Biochemicals , Signal Transduction
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