NMNAT1 ELISA Kit (Mouse)
Cat# OKWB00261
Size : 96Wells
Brand : Aviva Systems Biology
Datasheets/Manuals | Printable datasheet for NMNAT1 ELISA Kit (Mouse) (OKWB00261) |
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Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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Application | ELISA-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology Nmnat1 ELISA Kit (Mouse) (OKWB00261) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nmnat1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nmnat1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nmnat1 captured in the well. | ||||||||||||||||||||||
ELISA Kit Range | 15.625-1000pg/ml | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Mean Intra-Assay Precision <10% Mean Inter-Assay Precision <12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 9.4 pg/mL | ||||||||||||||||||||||
Assay Info | Assay Type: Quantitative Sandwich ELISA |
Gene Symbol | Nmnat1 |
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Gene Full Name | nicotinamide nucleotide adenylyltransferase 1 |
Alias Symbols | 2610529L11Rik;5730441G13Rik;D4Cole;D4Cole1e;naMN adenylyltransferase 1;nicotinamide mononucleotide adenylyl transferase;nicotinamide mononucleotide adenylyltransferase 1;nicotinamide/nicotinate mononucleotide adenylyltransferase 1;nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1;nicotinate-nucleotide adenylyltransferase 1;nm;NMN adenylyltransferase 1;NMN/NaMN adenylyltransferase 1;nmnat. |
NCBI Gene Id | 66454 |
Protein Name | Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1 |
Description of Target | Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP (PubMed:15381699). Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency (By similarity). Can use triazofurin monophosphate (TrMP) as substrate (By similarity). Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+) (By similarity). For the pyrophosphorolytic activity, prefers NAD(+) and NaAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively (By similarity). Involved in the synthesis of ATP in the nucleus, together with PARP1, PARG and NUDT5 (By similarity). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (By similarity). Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+) (By similarity). Protects against axonal degeneration following mechanical or toxic insults (PubMed:15310905, PubMed:16914673). Delays axonal degeneration after axotomy. Results in a >10-fold increase in intact neurites 72 hours after injury (PubMed:16914673). |
Uniprot ID | Q9EPA7 |
Protein Accession # | NP_597679 |
Nucleotide Accession # | NM_133435 |