Nitro blue tetrazolium chloride [298-83-9]
Cat# HY-15925-250mg
Size : 250mg
Brand : MedChemExpress
Nitro blue tetrazolium chloride (NBT) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures.
For research use only. We do not sell to patients.
Nitro blue tetrazolium chloride Chemical Structure
CAS No. : 298-83-9
This product is a controlled substance and not for sale in your territory.
Based on 4 publication(s) in Google Scholar
Description |
Nitro blue tetrazolium chloride (NBT) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures[1]. |
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In Vitro |
Protocol for Staining with NBT and BCIP MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Molecular Weight |
817.64 |
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Formula |
C40H30Cl2N10O6 |
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CAS No. |
298-83-9 |
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Unlabeled CAS |
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Appearance |
Solid |
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Color |
Light yellow to yellow |
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SMILES |
COC1=CC(C2=CC=C(N3N=C(C4=CC=CC=C4)N=[N+]3C5=CC=C([N+]([O-])=O)C=C5)C(OC)=C2)=CC=C1N6N=C(C7=CC=CC=C7)N=[N+]6C8=CC=C([N+]([O-])=O)C=C8.[Cl-].[Cl-] |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
4°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
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Solvent & Solubility |
In Vitro:
70%DMF : 100 mg/mL (122.30 mM; Need ultrasonic) H2O : 8 mg/mL (9.78 mM; Need ultrasonic) DMSO : 5 mg/mL (6.12 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Purity & Documentation |
Purity: 99.82% |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO / H2O / 70%DMF | 1 mM | 1.2230 mL | 6.1152 mL | 12.2303 mL | 30.5758 mL |
5 mM | 0.2446 mL | 1.2230 mL | 2.4461 mL | 6.1152 mL | |
70%DMF | 10 mM | 0.1223 mL | 0.6115 mL | 1.2230 mL | 3.0576 mL |
15 mM | 0.0815 mL | 0.4077 mL | 0.8154 mL | 2.0384 mL | |
20 mM | 0.0612 mL | 0.3058 mL | 0.6115 mL | 1.5288 mL | |
25 mM | 0.0489 mL | 0.2446 mL | 0.4892 mL | 1.2230 mL | |
30 mM | 0.0408 mL | 0.2038 mL | 0.4077 mL | 1.0192 mL | |
40 mM | 0.0306 mL | 0.1529 mL | 0.3058 mL | 0.7644 mL | |
50 mM | 0.0245 mL | 0.1223 mL | 0.2446 mL | 0.6115 mL | |
60 mM | 0.0204 mL | 0.1019 mL | 0.2038 mL | 0.5096 mL | |
80 mM | 0.0153 mL | 0.0764 mL | 0.1529 mL | 0.3822 mL | |
100 mM | 0.0122 mL | 0.0612 mL | 0.1223 mL | 0.3058 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Nitro blue tetrazolium chloride Related Classifications
- Others
- Fluorescent Dye
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
Nitro blue tetrazolium298-83-9NBTFluorescent DyedehydrogenasesBCIPwestern blottingimmunohistologicalInhibitorinhibitorinhibit