Nile Red [7385-67-3]
Cat# HY-D0718-100mg
Size : 100mg
Brand : MedChemExpress
Nile red (Nile blue oxazone) is a lipophilic stain. Nile red has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. Nile red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytof uorometry. Nile red stains intracellular lipid droplets red. The fluorescence wavelength is 559/635 nm.
For research use only. We do not sell to patients.
Nile Red Chemical Structure
CAS No. : 7385-67-3
This product is a controlled substance and not for sale in your territory.
Based on 55 publication(s) in Google Scholar
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Nile Red purchased from MedChemExpress. Usage Cited in: Nano Today. 47 (2022) 101675
- Subcellular biodistribution of NileRed-labeled PFD Liposome nano-particles in 4T1 and BT-549 cells are visualized by confocal laser microscopy. Blue, DAPI; Green, WGA 488; Red, NileRed-labeled PFD Liposome.
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Nile Red purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Oct 5;13(1):5871. [Abstract]
- Isolated oocytes are fixed with 4% paraformaldehyde for 1 hour at room temperature and washed 3 times with 1% BSA in PBS. Then, they are stained with 10 µg/ml Nile red at 4 °C overnight.
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Nile Red purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2022 Jun 24;50(12):6953-6967. [Abstract]
- The wild-type or G4-mutant human hepatic adenocarcinoma HepG2 cells are stained with Nile Red reagent to visualize the lipids with an Olympus Fluoview FV1000 confocal microscope.
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Nile Red purchased from MedChemExpress. Usage Cited in: EMBO J. 2022 Jul 4;e110439. [Abstract]
- Representative pictures of Nile red-stained human BAs differentiated from hESC-derived brown progenitors.
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Nile Red purchased from MedChemExpress. Usage Cited in: J Transl Med. 2022 May 14;20(1):222. [Abstract]
- Living cells are incubated with Nile Red (1 μM) for 15 min at 37 °C in dark. Then, cells are washed with HBSS/Ca/Mg and analyzed by fluorescence microscopy.
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Nile Red purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2022 Nov 24;175428. [Abstract]
- Hepatic lipid and FFA content are determined by Nile red staining of liver frozen sections.
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Nile Red purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Jan 1;11(5):2149-2169. [Abstract]
- Injected 5 μg of Nile Red lipid dye into the tail vein of mice to label triglycerides in the liver prior to intravital imaging.
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Nile Red purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2021 Sep 25;e13134. [Abstract]
- For lipid droplet staining, fixed cells are stained for 30 minutes with 0.1 µg/ml Nile Red (MCE), and the nucleus is counterstained using DAPI.
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Nile Red purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2021 May 31;22(11):5951. [Abstract]
- For lipid staining, fat bodies are submerged in Nile red solution at a final working concentration of 10 μg/mL in an acetone/water (1:9) mixture and visualized using a fluorescent microscope at Ex543/Em626 nm.
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Nile Red purchased from MedChemExpress. Usage Cited in: Nat Commun. 2020 Jan 13;11(1):240. [Abstract]
- HSCs are stained with Nile Red reagents and Bodipy493/503 to visualize the lipids with a light microscope.
Description |
Nile red (Nile blue oxazone) is a lipophilic stain. Nile red has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. Nile red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytof uorometry. Nile red stains intracellular lipid droplets red. The fluorescence wavelength is 559/635 nm[1]. |
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In Vitro |
1. Preparation of Phalloidin-TRITC working solution MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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In Vivo |
When Nile red-stained Caenorhabditis elegans is viewed for green fluorescence, discrete lipid bodies can be observed throughout the intestine and other tissues either in clusters or evenly dispersed, depending on the animal's genotype or experimental treatment[3]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Molecular Weight |
318.37 |
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Formula |
C20H18N2O2 |
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CAS No. |
7385-67-3 |
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Appearance |
Solid |
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Color |
Green to dark green |
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Emission (Em) |
598 |
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Excitation (Ex) |
543 |
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SMILES |
O=C1C2=CC=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4OC3=C1 |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
4°C, protect from light *In solvent : -80°C, 2 years; -20°C, 1 year (protect from light) |
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Solvent & Solubility |
In Vitro:
DMSO : 2 mg/mL (6.28 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Ethanol : 1 mg/mL (3.14 mM; ultrasonic and warming and heat to 60°C) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Purity & Documentation |
Purity: 98.15% |
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Dyeing Example |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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Ethanol / DMSO | 1 mM | 3.1410 mL | 15.7050 mL | 31.4100 mL | 78.5250 mL |
DMSO | 5 mM | 0.6282 mL | 3.1410 mL | 6.2820 mL | 15.7050 mL |
Nile Red Related Classifications
- Others
- Fluorescent Dye